The Rate of Hydrolysis of Sucrose Essay -- Biology Science Experiments

The Rate of Hydrolysis of Sucrose Hypothesis - The digestion of sucrose into glucose and fructose is carried out by the presence of the enzyme Sucrase. The activity of the enzyme will be greatest at its optimum temperature since at this temperature the enzyme's tertiary structure will be correct to allow the formation of the substrate-enzyme complex quickly and efficiently. This should be at 37Â °C (body temperature), which is the temperature of the ileum where Sucrase is secreted and naturally functions. The first thing to say about enzymes is that they are proteins and they are found in all types of organisms from humans to viruses. They function in the body as catalysts. In other words they speed up the rate of chemical reactions in the body. Enzymes actually accelerate chemical reactions by a factor of about 1 million. Enzymes are organic catalysts. Without enzymes the metabolism of an organism would be too slow for the organism to survive. The word enzyme actually means "in yeast" as they were first discovered in these micro-organisms. Enzymes are found inside cells (intracellular) and outside cells (extra cellular). Intracellular enzymes are for example, those that control metabolism while extra cellular enzymes are for example, gut digestive juices and the enzymes secreted by bacteria, which digest their food outside the body then reabsorb the products. Enzymes work on particular organic chemicals, these are called substrates. So in effect food is the substrate of digestive enzymes. Each enzyme is specific to one or a group of particular substrates. The majority of the reactions that occur in living organisms are enzyme-controlled. Without enzymes toxins would soon build up and the supply of respiratory substrate would decrease. Enzymes are proteins and thus have a specific shape. They are therefore specific in the reactions that they catalyse - one enzyme will react with molecules of one substrate. The site of the reaction occurs in an area on the surface of the protein called the active site. Since the active site for all molecules of one enzyme will be made up of the same arrangement of amino acids, it has a highly specific shape. Generally, there is only one active site on each enzyme molecule and only one type of substrate molecule will fit into it. Lock and key hypothesis description of the specificity of t... ... well as this the final colour was defined. Results : my results from then on were much more reliable and the average was easier to plot on a graph drawing my expected curve. The first colour change was a loss of the base pink colour and the final colour change is a measurable blue. Throughout all these preliminary experiments and the final one all laboratory safety precautions will be observed. To check the reliability of my results I will take a mean average of all 3 experiments I finally did. Using the mean of all the experiments I then will work out the standard deviation of the primary colour change and the final colour change. On the graphs the X axis is numbered 1-6, this corresponds to the Sucrase Concentration 0.0%-2.5%. Below is the preliminary experiment graph for primary colour change at 0-5% solution. The line slopes down as the reaction time decreases in relation to the concentration. This was the graph of the preliminary experiment after making the changes due to evidence from preliminary experiment 1 and 2. Prediction: I predict that the higher the concentration the faster the reaction due to induced fit and lock and key hypothesis.